Revolutionary holotomographic
(3D holographic) microscopy opens new era for label-free live cell imaging

Cellular analysis plays a crucial role in a wide variety of research and diagnostic activities in the life science. However, the information available to researchers and clinicians is limited by current microscopy techniques. An innovative new tool – Holotomographic microscopy – can overcome many of these limitations and open new vistas for researchers and clinicians to understand, diagnose and treat human diseases.

Holotomographic Microscopy – New era of microscopy Tomocube’s holotomography series utilize optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate biological cells and thin tissues. ODT reconstructs the 3D refractive index (RI) distributions of live cells and by doing so, provides structural and chemical information about the cell including dry mass, morphology, and dynamics of the cellular membrane.


TomoStudio™, Software platform for 2D/3D/4D image analysis

TomoStudio™, HT-1 operating SW, controls the system and visualizes the captured image in various ways. This flexible user interface provides fast imaging capability and 2D/3D/4D visualization of the cellular image based on 3D RI distributions of the cells and tissues. follow this link. TomoStudio™

Smart technology for holotomography

HT-1 use holotomography technology which measures the 3D and 4D refractive index (RI) tomograms. This new technology enables quantitative, label-free measurements of live, unstained cells and tissue samples. With the dynamic micromirror device (DMD), we have eliminated moving parts from light path while image-capturing and has added unprecedented stability and precision on the outstanding performance of our product. If you want to get more information about our technology, follow this link. Technology

Features and benefits

Key Features

3-D Refractive Index:
new imaging contrast

No labeling

High resolution

Low laser

Fast in 2D/3D

High impact, Low cost


Zero stress
Label-free imaging

Intact live cell imaging
Long-term image with short time interval

Time saving
No sample preparation and Rapid 3D cell imaging

High quality 3D
Optical resolution below 200 nm (Max. 110 nm)

Quantitative bioimaging
RI enables quantitative bioimaging
(local cytoplasmic concentration, dry mass)

Technical specification

Environmental requirement

HT-2 : Holotomographic microscopy with 3D fluorescence imaging capability

Research and diagnosis in the life sciences depends on the information that can be found using cellular analysis. However, current microscopy techniques limit the quantity and quality of information available to researchers and clinicians

The HT-2, a microscope that displays both 3D holograms and 3D fluorescence imaging, is able to overcome these limitations through use of new and existing technology. Its abilities can enable researchers and clinicians to break into new frontiers of understanding, diagnosing, and treating disease.
HT-2 is the world’s first microscopy combining both holotomography and 3D fluorescence imaging in one unit.

The HT-2 is an update to our HT-1 microscope. The HT-1 microscope uses holotomographic technology to measure the 3D and 4D refractive indexes using a dynamic micromirror device (DMD) to add unprecedented stability and precision to the performance of the microscope. These innovations allow for quantitative, label free imaging of cells. The HT-2 has all the capabilities of the HT-1, but combines the holotomographic imaging capabilities of the original version with the added ability to perform fluorescence microscopy.



3D fluorescence

3D fluorescence
(DAPI, GFP-mitochondria)

3D RI tomogram + 3D fluorescence

3D RI tomogram 

3D RI tomogram

3D RI tomogram +
3D fluorescence

2D fluorescence (DAPI, GFP)

2D fluorescence


Auto fluorescence - Blue

Auto fluorescence – Blue

Auto fluorescence - Green

Auto fluorescence – Green

Auto fluorescence - Red

Auto fluorescence – Red

RI tomogram (XY cross section)

RI tomogram
(XY cross section)




GFP - tubulin

GFP – tubulin



RI tomogram (XY cross section)

RI tomogram
(XY cross section)

Features and benefits

Key Features

A three-channel LED light source
(385 nm, 470 nm, 570 nm)

Wavelengths of the LED source can be customized

Z-stack images with a motorized Z-drive (step resolution: 150 nm)

Correlative analysis in 2D, 3D and 4D with HT and fluorescence images

Upgradable: The HT-1 can be upgraded easily to the HT-2(fluorescence version) in the field



Correlative microscopy in one instrument
HT-2 provides high-quality 3D images of both holotomography and 3D fluorescence for each sample.


Quantitative data marked with fluorescence
HT-2 provides morphological (volume, surface area, projection area, sphericity and ellipticity), chemical (dry mass, concentration) and mechanical (cell deformability) properties of cells with 3D refractive index (RI) tomogram. Moreover, fluorescence image provides information about molecular specificity.


Live cell molecular and holographic imaging with minimal stress on cells
Simultaneous measurement capability of time-lapse 3-D RI tomography and fluorescence image allows long-time tracking of specific targets in live cells. The fluorescence image provides the position of specific target organelles or structures in live cell, and consecutive measurements of time-lapse 3-D RI tomography enables the monitoring of cells and their structures with minimal stress.

Working scenarios

Scenario 1

Fast 2D fluorescence (FL) + 2D holography: enables the observation of very fast dynamics in the living cell through 2D holographic images (150 fps) after viewing the 2D FL image.

Scenario 1 - Cycle 1
Scenario 1 - Cycle 2

Scenario 2

Fast 2D FL + 3D HT: using a single 2D FL image, mark your target in the living cell and follow it across time using 3D HT images, without damaging the cell.

Scenario 2 - Cycle 1
Scenario 2 - Cycle 2

Scenario 3

3D FL + 3D HT: It is possible to obtain 3D FL and 3D HT images simultaneously, which allows researchers to investigate the 3D morphology of the cell with unprecedented imaging modalities. The quality and resolution of the 3D FL image can also be enhanced by using a deconvolution software.

Scenario 3 - Cycle 1
Scenario 3 - Cycle 2

HT-2 fluorescence specification

If you need customization for HT microscope, please contact us.