TomoStudio™, easy to use interface of HT series.

TomoStudio™ works conjunctively with our HT models, to visualized and analyze RI tomograms.
With TomoStudio™, an user can color code the image according to refractive index and identify various types of quantitative data.

TomoStudio™ provides

Work flow interface
User interface allows uninterrupted workflow from operating the microscope to analyzing acquired datasets.

Data backup
Raw data can be stored in the computer for further analysis.

Fast image acquisition
HT captures Holotomographic images every 0.4 second (2.5 f/s) and 2D holographic images every 0.007 second (150 f/s).

Data analysis
Data can be processed quantitatively in real-time. User can also utilize various quantitative analysis methods.

Holographic staining
Digital color coding controller (Transfer function) is a graphical user interface to digitally stain a sample based on its refractive index information.

Dynamic image processing
Data processing does not interfere with the image acquisition process. Selective data processing is possible at any moment.

Motorized stage function
& Mark and find
Multiple cell positions can be recorded and recalled using our motorized stage and mark & find function.

Click and shift
Just by clicking the mini-map, the user can control the movement of the sample stage.

Output parameters

Morphological parameter

Volume (㎛³)
Surface Area (㎛²)
Projection Area (㎛²)
Sphericity

Chemical parameter

Dry mass (pg)
Concentration (pg/㎛³)
*RBC: Hb contents and concentration
*Red Blood Cell

Mechanical parameter

Cell stiffness
Dynamic membrane fluctuation

TomoStudio™ 2.0 optimized for the HT-2 series

Features

Obtaining 3D RI Tomogram
Obtaining 3D fluorescence images up to 3 colors
‘Mark’ and ‘Find’ function for recoding the position of multiple cells
Control of fluorescence and tomogram with different time series
(Hetero time-lapse: e.g. 1 FL in every 10 tomogram)

Visualization

1

Digital color coding of RI tomogram with high flexibility

2

Gradient transfer function displays the additional differentiation factor of RIs in the cell

3

Digital 3D overlay of
FL and HT images

4

Improved FL resolution with the deconvolution method

Stitcher

Now the dataset acquisition and analysis for large areas become possible with our tile & matrix scanning function in Tomostudio™ and the specialized stitching algorithm in Stitcher.

Our automated stage scans the specimen up to 8 mm × 8 mm area in designated sized tiles or matrices. These 3D tiles and matrices are then stitched together using Stitcher. Our technology enables users to image their specimens in a larger view with high optical resolution, giving new insights to their researches.

Bacterial Cell Counter

Bacterial Cell Counter provides a simple and time-saving way to automatically count the colony-forming-unit (CFU) of bacterial culture.

Users can acquire multiple tomograms of their samples by using HT series and Tomostudio™’s automatic matrix-scanning function. Bacterial Cell Counter then counts the number of spores in the 3D tomograms and extracts quantitative parameters, such as average volume and average dry mass.
Accuracy of counting is comparable to that of conventional agar plating method, while additional preparation steps, such as staining and agar plating, are not required.

Lipid Analyzer

Lipid Analyzer helps users quantitatively analyze lipid contents in their samples by automatically sorting and analyzing lipid droplets in the 3D tomograms.

Users can define maximum and minimum size, as well as refractive index threshold of their targeted lipids.
Lipid Analyzer then automatically detects inside the images and produces physical parameters of lipid contents, including volume and mass.
With Lipid Analyzer, users will find a precise and efficient way to analyze the lipid contents in their samples.

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