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Tomocube, Inc.
Realtime 3D Label-free Cell Imaging and Visualization
Tomocube, Inc.
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  • Products
    • HT-X1
    • HT series
    • TomoStudio™
    • TomoAnalysis™_oldpage
    • Accessories
    • Industrial HT
  • Technology
    • Holotomography
    • Tomocube AI
    • FAQ
  • Applications
    • Organoids Analysis
    • Lipid Quantification
    • Bacteria Analysis
    • Phase Separation
    • Cell Library
  • Resources
  • Publications
  • About
    • Media
    • News
    • Distributors
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Applications

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Fixed Human Liver Organoid

Organoids Analysis

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Lipid quantification

Lipid Quantification

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Bacterial analysis

Bacteria Analysis

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Phase separation

Phase Separation

Cell Library

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Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Cell division

Cell division

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Mouse neuron

Mouse neuron

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_RICE-Pollen

Pollen

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_HeLa cell_Correlative Imaging HT FL

HeLa

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_schwann

Schwann

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Osteoblast

Osteoblast

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Cell division

Cell division

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_NIH3T3

NIH3T3

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Color-coded projection of HT image

In order to improve the visualization of our 3D organoid structure in label-free imaging, we employed an adaptation of the Sobel image filter – a well-known image processing technique for accentuating edges in an image.

Specifically, we filtered the gradient images produced by the Sobel filter and color-coded them according to their depth. Subsequently, we projected the maximum value of the resulting colored gradient image in the z direction.

This methodology enabled us to gain a more detailed and informative representation of our 3D organoid structure.