Label-Free 3D High-Resolution Imaging of Live Primary Neuronal Cells and Fixed Mouse Brain Structures Using Low-Coherence Holotomography
Applies low-coherence holotomography to two neuroscience imaging challenges: real-time monitoring of live primary rat neuron dynamics (axon growth, mitochondrial transport) and structural delineation of 200-µm-thick live mouse brain tissue sections at single-axon resolution, without staining.
What's inside
- Real-time imaging of live primary rat neuronal cells, resolving soma, nucleus, dendrites, and axon; time-lapse tracking of growth-cone lamellipodium dynamics
- 3D imaging of a 200-µm-thick live mouse brain coronal section, annotated across cerebral cortex, hippocampus (CA1, dentate gyrus), retrosplenial cortex, and additional subregions
- Architectural-layer-level detail in hippocampal CA1 (stratum oriens/radiatum/pyramidale/lacunosum moleculare) and dentate gyrus (polymorph/molecular/granule cell layers)
- Discussion of HT's potential for neural network formation studies and neurological disease research