Cell-to-cell Interaction in the Immune Response

Cell-to-cell interactions in the immune response are rapid and highly dynamic, making them difficult to study continuously with conventional fluorescence methods that cause phototoxicity. Holotomography captures these interactions label-free in 3D with high temporal resolution, allowing quantitative tracking of synapse formation, membrane dynamics, and biochemical changes over time. This note also demonstrates how HT can be combined with deep learning for automated, high-throughput analysis.

What's inside

- Label-free time-lapse imaging of immunological synapse formation between CAR-T cells (CART19) and CD19+ cancer cells (K562-CD19)

- Quantification of IS contact area kinetics and membrane protein density over time using RI values

- Correlative HT and fluorescence imaging for validating deep-learning-based IS segmentation (DeepIS)

- NK cell–cancer cell interaction imaging including lytic granule release and target cell death

Keywords

immunological synapse CAR-T cell immune response label-free cell interaction holotomography NK cell T cell imaging cancer immunotherapy live cell imaging time-lapse microscopy membrane protein density deep learning cell segmentation

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