Label-free Quantification of Intracellular Lipids

Lipid droplets have distinctly high refractive index values, making them naturally visible in holotomographic images without any staining. HT enables label-free 3D quantification of lipid content in live cells, supporting time-lapse studies of metabolic dynamics. Applications span liver disease research, metabolic studies, and biofuel-relevant microalgae biology.

What's inside

- Label-free 3D imaging and quantification of lipid droplets in live human hepatocytes (Huh-7), validated against Nile Red staining

- Measurement of lipid droplet volume, mass, and distribution following oleic acid treatment

- Lipid content quantification in single microalgae (Nannochloropsis oculata) under nitrogen deficiency

- Correlative HT and fluorescence imaging using HT-2H for co-localization studies

Keywords

lipid droplet imaging label-free lipid quantification intracellular lipid holotomography refractive index tomography hepatocyte imaging fatty liver metabolic disease microalgae biofuel time-lapse live cell imaging lipid metabolism 3D RI tomogram

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