Lipid Quantification
Compared to that of protein, lipids have a uniquely high refractive index increment (RII) value which can be used to distinguish them from most other cell compartments in terms of RI distribution.
HT is a better way to analyze lipid
Label-free
HT imaging eliminates pre-treatment process and saves time
Accurate
Lipid volume and weights are calculated based on 3D refractive index values
In situ single cell imaging
Lipids within cells are imaged and calculated at a single-cell level
Live-cell
No phototoxicity from fluorescent probes means cells remain healthy for even long-term imaging
Lipid segmentation and quantification in Tomoanalysis
Application: Quantifying lipid accumulation in diseases
Publication
Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy
Seungeun Oh, ChangHee Lee, […], Marc W. Kirschner, The Proceedings of the National Academy of Sciences (2022)
Label-Free Tomographic Imaging of Lipid Droplets in Foam Cells for Machine-Learning-Assisted Therapeutic Evaluation of Targeted Nanodrugs
Sangwoo Park, Jae Won Ahn […] YongKeun Park, Seongsoo Lee & Kyeongsoon Park, ACS Nano (2020)
Three-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
Kyoohyun Kim, SeoEun Lee […] YongKeun Park, Scientific Reports (2016)