Organoid Imaging and Analysis

Real-time Imaging of Organoids with Holotomography

Unlock the Full Potential of Organoids with HT

Holotomography imaging – the game changing tool for precision medicine and drug discovery

Label-free imaging

HT imaging does not require any labeling or staining, therefore provides more reliable and relevant information.

High resolution 3D imaging

HT generates high quality, meaningful data by resolving spatial and long-term, temporal detail.

Timelapse imaging

HT allows real-time imaging of organoids in their natural states, enabling the observation of their behavior and dynamic over time.

Downstream analysis

After HT imaging, researchers can return live organoids sample to the incubator for further observation or downstream analysis.

Simplified sequential workflow

HT enables more efficient experiments by reducing time-consuming preparation steps and chemicals used.

Cost effective

HT reduces the general cost of experimentation by minimizing labor cost, time expense and sample damages.

3D Organoid Imaging and Analysis Workflow

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Organoids Simplified Workflow

Tomocube HT-X1 - Extra features for your best outcome

Fluorescence microscope

Tomocube HT-X1

High resolution 3D imaging

Fluorescence imaging


Subcellular structure visualization

Minimal sample toxicity

Non invasive imaging

Downstream analysis

convenient workflow

3D Imaging of Organoids

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Human Lung Organoids Snap shot

Human Lung Organoid

Fixed lung organoid captured by the HT-X1. The image was taken after the organoid was resuspended in PBS, placed at Tomodish and covered with a coverslip.

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Fixed Human Liver Organoid

Human Liver Organoid

Fixed human liver organoid captured by the HT-X1. Diverse subcellular-level features of individual cells such as nucleus, nucleoli, mitochondria, vacuoles, and granules are visible.

Mouse Small Intestinal Organoid

The 3D structure of a mouse small intestinal organoid was captured by HT-X1. This video shows diverse microscale features the intestinal organoid such as lumen, epithelium, paneth cells, and goblet cells.

Mouse Small Intestinal Organoid

Label-free imaging video of a mouse small intestinal organoid growing in Matrigel for 5 days in its native state. Holotomography video was post-processed to enhance visualization by color-coded projection technique.

Quantitative Analysis

Tomocube_Label-free 3D Live Cell Imaging_Holotomography_Quantitative analysis of organoid growth in 5 days

Quantitative analysis of organoid growth for 5 days

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