Label-free
HT offers visualization of liquid-liquid phase separation without markers
Quantification
Protein condensate mass can be measured based on the refractive index (RI)
3D visualization
Liquid droplet formation in live cells can be viewed in 3D
Simultaneous HT and fluorescence imaging
HT-2H offers HT and fluorescence imaging to offer you unrestricted design for your experiments
Publications using HT
Published in Nature cell biology cover page (March 2021)
TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis
Nat Cell Biol 23, 257–267 (2021).
Holotomographic images and quantitative analysis of the aggresomes
Aggresomal sequestration and STUB1-mediated ubiquitylation during mammalian proteaphagy of inhibited proteasomes
PNAS 117 (32) 19190-19200 (2020)
Publication
TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis
Mark Esposito, Cao Fang, […] , Ileana M. Cristea & Yibin Kang, Nature Cell Biology (2021)