Example images of microscopes
This is the principle utilized in Phase Contrast and Differential Interference Contrast microscopy, techniques that are widely used to image unstained biological samples. However, these methods image in two dimensions while biological samples are actually three dimensional. The Tomocube HT solves this problem. When we combine the image data from the beam passing through the sample with the reference beam we create a hologram image. By rotating the imaging beam through 360° we can capture a sequence of holograms from different angles. The resulting 2D hologram images of a sample obtained with various illumination angles are reconstructed into a 3D RI tomogram. Cellular Holotomography is analogous to X-ray computed tomography (CT) and magnetic resonance imaging (MRI). Unlike phase contrast microscopy, HT provides quantitative (RI-based) and 3D tomographic reconstruction capability.