HoloTomography(HT) is a laser interferometric technique which is employing a method broadly analogous to the use of X-rays in CT scans.
In order to improve the visualization of our 3D organoid structure in label-free imaging, we employed an adaptation of the Sobel image filter – a well-known image processing technique for accentuating edges in an image.
Specifically, we filtered the gradient images produced by the Sobel filter and color-coded them according to their depth. Subsequently, we projected the maximum value of the resulting colored gradient image in the z direction.
This methodology enabled us to gain a more detailed and informative representation of our 3D organoid structure.