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A full 3D refractive-index volume plus quantitative derived values — critical dimensions, depth and taper, step heights, surface and sidewall roughness, and defect locations — viewable and exportable in TomoAnalysis MI software.
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Send us a sample under NDA. We measure it free of charge, prepare a 3D analysis report, and review the dataset live with you. Use the Inquiry button or email precision@tomocube.com.
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Both. The Module configurations are designed for integration into industrial inspection setups (with TomoPilot host interface), while Desktop configurations suit lab and pilot-line use with motorized stages and analysis software.
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Transmission systems (HT-T1™) image inside transparent materials such as glass up to 2 mm thickness. Stage travel and maximum panel size depend on configuration — contact us with your sample dimensions for a specific answer.
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A single field-of-view acquisition takes seconds to minutes depending on volume depth and resolution settings. Larger areas are covered by automated stage stitching. For throughput estimates on your structure, request a sample measurement.
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No. Holotomography is label-free and contact-free — no cutting, polishing, coating, or staining. Samples are measured as-is and remain fully intact afterwards, so the same part can continue through your process or be re-measured later.
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Holotomography (HT), analyzed using software like TomoAnalysis, provides a wealth of information across multiple parameters. These include morphological, chemical, and mechanical aspects of the imaged samples.
Morphological Parameters: HT enables the measurement of various morphological parameters such as volume, surface area, projection area, and sphericity. These parameters offer insights into the shape and size characteristics of the imaged objects.
Chemical Parameters: Through holotomographic analysis, chemical information such as dry mass and concentration can be determined. For example, in the case of red blood cells, hemoglobin concentration can also be measured, providing valuable biochemical insights.
Mechanical Parameters: HT facilitates the measurement of mechanical properties such as cell stiffness. This parameter offers crucial information about the mechanical behavior and integrity of the imaged samples, which can be particularly relevant in studying cellular biomechanics and response to external stimuli. -
Phase contrast and Differential Interference Contrast (DIC) microscopy are widely used techniques for visualizing unstained live cells with high contrast. However, both phase contrast and DIC microscopy offer only 2D qualitative imaging.
Holotomography (HT), on the other hand, provides quantitative 3D tomograms. This means it not only captures the structural details of the sample but also allows for precise measurements of various parameters within the sample volume. Unlike phase contrast and DIC microscopy, which rely on contrast enhancements, HT directly measures the refractive index variations within the sample, enabling detailed three-dimensional reconstructions. This quantitative aspect of HT makes it particularly valuable for studying dynamic biological processes and understanding cellular structures in greater detail.